BACKGROUND AND OBJECTIVES Bacillus anthracis is one of the most homogenous bacteria ever described. Some level of diversity. Bacillus anthracis 17JB is a laboratory strain It is broadly used as a challenge strain in guinea pigs for potency test of anthrax vaccine. MATERIAL AND METHODS This work describes genetic characterization of B. anthracis 17 JB strain using the SNPs and MLVA genotyping....

background and objectives: bacillus anthracis is one of the most homogenous bacteria ever described. bacillus anthracis 17jb is a laboratory strain. it is broadly used as a challenge strain in guinea pigs for potency test of anthrax vaccine. material and methods: this work describes genetic characterization of b. anthracis 17 jb strain using the snps and mlva genotyping. results and conclusion:...

Virulent typical strains (Shikan, Morioka, Shizuoka) and Pasteur vaccine strains (no. 1, no. 2-H, no. 2-17JB) of Bacillus anthracis harboured two plasmid species with molecular masses of 110 MDal and 60 MDal. All of the 110 MDal plasmids isolated from the various strains showed indistinguishable patterns of digestion with restriction endonucleases. All the 60 MDal plasmids were also indistingui...

background: bacillus anthracis may usually cause three forms of anthrax: inhalation, gastrointestinal and cutaneous. the gastrointestinal (gi) anthrax develops after eating contaminated meat. thus, in this paper were report 5 cases of intestinal anthrax. case presentation: we report a case series of intestinal anthrax, with history of consumption of raw or poorly cooked liver of sheep. one pati...

Keywords: Bacillus anthracis, diagnostics, identification, rapid tests, microbiological studies, methods of molecular microbiology

There have been many efforts to develop Bacillus anthracis detection assays, but the problem of false-positive results has often been encountered. Therefore, to validate an assay for B. anthracis detection, it is critical to examine its specificity with the most closely related Bacillus isolates that are available. To define the most closely related Bacillus isolates to B. anthracis in our Baci...

INTRODUCTION Bacillus anthracis is the causative agent of anthrax, a disease endemic in regions of Northern Cape Province and Kruger National Park of South Africa. Accurate identification of virulent B. anthracis is essential but challenging due to its close relationship with other members of B. cereus group. This study characterized B. anthracis and Bacillus species that were recovered from an...

A fluorescein-conjugated antibody against formalin-inactivated spores of Bacillus anthracis Vollum reacted only weakly with a variety of Bacillus species in microfluorometric immunofluorescence assays. A conjugated antibody against spores of B. anthracis Sterne showed little affinity for spores of several B. anthracis isolates including B. anthracis Vollum, indicating that more than one anthrax...

to isolate and detect anthrax spores from soil in different regions of isfahan, iran a total of 60 environmental specimens were collected during 2003. bacterial endospores were extracted via flotation in distilled water and were cultured on blood agar and selective plet media. bacillus anthracis was identified using bacteriological and biological tests. viable bacillus anthracis spores were iso...

abstract snp typing is now a well-established genotyping system in bacillus anthracis studies. in the original standard method of van erth, snps at 13 loci of the b. anthracis genome were analyzed. in order to simplify and make appropriate this expensive method to low-budget laboratory settings, 13 primer pairs targeting the 13 corresponding snps were designed. besides, a universal pcr protocol...